| Time | Hole | Players |
|---|---|---|
| 1A |   Olivia LangeWilliam Penn University  Micaela SuhlPark University   Reagan KauthBaker University  Sophia HoneymanMount Mercy University | |
| 1B |  Gabriana FrancoWilliam Penn University Anna KorsenPark University  Paula Martin-MonteroBaker University Carli IrvineMount Mercy University | |
| 2A |  Emma LawsonWilliam Penn University Kim AlbertusPark University  Izabelle BäckströmBaker University  Grace HaasMount Mercy University | |
| 2B |  Riley BurnsWilliam Penn University Alexandra CaglePark University  Ally LavertyBaker University  Kyla JohnsonMount Mercy University | |
| 3 |  Bliss MartinWilliam Penn University Grace RogersPark University  Madison ZubeckBaker University Lauren McPhillips-NewcombMount Mercy University | |
| 4 |   Megan RiesselmanGrand View University   Hanna RiekerMissouri Valley College   Amy FusedaleMidAmerica Nazarene University  Jamie Alex BarnettPeru State College | |
| 5 |  Kate RahfeldtGrand View University  Kendra WardenMissouri Valley College Maeva FardelMidAmerica Nazarene University Chloe ClappPeru State College | |
| 6 |  Jaya WestGrand View University  Madison MillsMissouri Valley College Adrianna AdamsMidAmerica Nazarene University Olivia McAseyPeru State College | |
| 7 |  Dani HurtGrand View University Sydney RighterMissouri Valley College Laurel RepkaMidAmerica Nazarene University Maddie KimePeru State College | |
| 8 |  Jessi WagnerClarke University Victoria Menard VarelaMissouri Valley College Karlie ChipmanMidAmerica Nazarene University Abigail MathisPeru State College | |
| 9A | Irene MetaxasClarke University  Sarah GutuzaWilliam Penn University (IND) Maya EnglandClarke University | |
| 9B | Livia HermansonClarke University  Caitlin ScanlonBaker University (IND) |
